Xenbase Image ID: 74273

Fig.6. Suppression of Notch signaling during gastrulation results in the robust expression of cardiac field genes. Gastrula 11–11.5 stage embryos are oriented laterally, vegetal to the left. (A, C) During gastrulation, cardiac field marker transcripts nkx2.5 and tbx5 are not detected by in situ hybridization analysis in uninjected embryos. (B, D) One blastomere of cleavage-stage embryos was injected with mRNAs encoding GR-Su(H)DBM plus a lineage marker. At the onset of gastrulation (stg. 10) constructs were induced via the addition of hormone. When Notch signaling was downregulated during heart field specification, the expression of both nkx2.5 (B; 76% of embryos, n =  117) and tbx5 (D; 79% of embryos, n =  86) robustly expressed in the migrating cardiac precursor cell population, whereas expression in uninjected embryos is difficult to detect via in situ hybridization at these stages (A, C). Black arrows denote the injected side of embryos. Percents are based on the proportion of embryos with the molecular phenotype indicated across triplicate experiments performed on three separate days. Image published in: Miazga CM and McLaughlin KA (2009) Copyright © 2009. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue nkx2-5.L AR2, csx, Nkx-2.5, nkx-2.5, nkx2-5-a, nkx2-5-b, nkx2.5, tinman, XNkx-2.5, XNkx2-5 X. laevis Throughout NF stage 11.5   tbx5.S tbx-5, tbx5-a, tbx5-b, xtbx-5, xtbx5 X. laevis Throughout NF stage 11   Image source: Published Larger Image Printer Friendly View

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