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Xenbase Image ID: 74403

Fig. 4. Ionocytes express the transcription factor foxi1e. (A) Whole-mount in situ hybridisation and cross-sections of stage 14 and stage 25 embryos hybridised with foxi1e probe. foxi1e is expressed in a spotted pattern and the location of the expressing cells changes from the inner to outer layer between the neurula and tailbud stages. Sections are on fluorescent in situ samples with foxi1e in red and DAPI staining in blue. Scale bars: 250 μm (whole mounts); 50 μm (sections). (B) Whole-mount double immunostaining of stage 27 tadpoles with anti-foxi1e, anti-acetylated tubulin and anti-xeel, as indicated. foxi1e-expressing cells (green) are distinct from ciliated (CC) and goblet (GC) cells. Scale bars: 50 μm. (C) Whole-mount in situ hybridisation with foxi1e probe and immunostaining with anti-ca12 and anti-v1a, in the combinations indicated. Upper and lower panels represent the same field of imaging, with upper panels showing the in situ probes and lower panels showing antibody staining overlaying the in situ probes. In the foxi1e/ca12 combination, arrows point to three cells that express foxi1e but not ca12, and arrowhead points to a cell that expresses both. In the foxi1e/v1a combination, complete overlap is detected. In the v1a/ca12 combination, arrows point to three cells that express v1a but not ca12, and arrowhead points to a cell that expresses both. Scale bars: 40 μm.

Image published in: Dubaissi E and Papalopulu N (2011)

Image downloaded from an Open Access article in PubMed Central. © 2011. Published by The Company of Biologists Ltd

foxi1 Xema, ectodermally-expressed mesendoderm antagon, FoxI1e, xfoxi1, ema, foxi1e, foxi3X.tropicalisSometime during NF stage 14 to NF stage 25epidermis

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