Xenbase Image ID: 74469

Fig. 2. Vangl2 protein distributes with and maintains the acetylated microtubule cytoskeleton. (A) Co-immunostaining of sections from uninjected and Vangl2-V5 mRNA (20 pg)-injected stage 6 Xenopus oocytes using Vangl2 (VG) and V5 tag (V5) antibodies. (B) Co-immunostaining of sections of wild-type stage 6 oocytes for acetylated tubulin (aTub) and Vangl2 (VG) in the animal and vegetal hemispheres. (C) Co-immunostaining for acetylated tubulin and Vangl2 protein in sections of nocodazole-treated (5 μg/ml) and taxol-treated (2 μM/ml) stage 6 oocytes. (D) Optical sections (2.6 μm thickness) of stage 6 control and Vangl2-depleted (VG–) oocytes stained as whole mounts to show the acetylated tubulin cytoskeleton. Insets show higher magnification images of histological sections stained with the same antibody. Right-hand panel shows a control whole mount stained with secondary antibody only. Image published in: Cha SW et al. (2011) Copyright © 2011. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue vangl2.L lpp1, ltap, stb1, stbm, stbm1, strabismus, Van Gogh, van gogh-like2, vang, vang-like 2, vangl2-a, vangl2-b, Xstrabismus X. laevis Throughout oocyte stage VI animal hemisphere vegetal hemisphere tuba8.S tubal2 X. laevis Throughout oocyte stage VI animal hemisphere vegetal hemisphere Image source: Published Larger Image Printer Friendly View

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