Xenbase Image ID: 74471

Fig. 4. Vangl2 is required for VAMP1 protein levels. (A) Diagram showing the procedure for generating the z-stack images shown in B and C. (B,C) z-stacks of the entire animal hemisphere projected as a single image for control (B, Uninjected) and Vangl2-depleted (C, VG–) stage 6 Xenopus oocytes, co-immunostained for VAMP1 and Vangl2 (VG) proteins. Chevrons point to areas that are devoid of both VAMP1 and Vangl2. (D) Western blot of lysates from stage 6 control (Un) and Vangl2-depleted (VG–) oocytes probed for VAMP1 and Vangl2. Antibodies against alpha-tubulin and Xenopus Disheveleds (Dvls) are used as loading controls. Quantification is shown for VAMP1 versus alpha tubulin for this experiment. The experiment was repeated with a similar result (*P<0.02, determined by t-test). Error bars represent s.d. (E) Co-immunoprecipitation analyses showing that endogenous Vangl2 protein interacts with VAMP1 in control (Uninj) and nocodazole (Noco)-treated stage 6 (St. VI) oocyte lysates, and that the interaction is increased by taxol treatment. Image published in: Cha SW et al. (2011) Copyright © 2011. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue vamp1.L syb1, synaptobrevin, synaptobrevin 1, synaptobrevin1, vamp-1 X. laevis Throughout oocyte stage VI animal hemisphere vangl2.L lpp1, ltap, stb1, stbm, stbm1, strabismus, Van Gogh, van gogh-like2, vang, vang-like 2, vangl2-a, vangl2-b, Xstrabismus X. laevis Throughout oocyte stage VI animal hemisphere Image source: Published Larger Image Printer Friendly View

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