XB-IMG-74478
Xenbase Image ID: 74478
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Fig. 4. Visualization of growing MT plus ends via GFP-EB1 in extracts with control antibody (Left) or anti-Dgt4 antibody (Right). (A) Representative images of tubulin (Cy3-channel) and GFP–EB1 (140 nM) are shown for extract incubated with control or Dgt4 antibody (see Fig. 3G and Fig. S3 C–E) at three assembly stages (see Fig. 1D). (B and C) Augmin-inhibited spindles show a reduction of EB1–GFP comets from the middle of the spindles. Note the prominent density of EB1–GFP comets at the acentrosomal poles in C (both control and Dgt4 AB). (Scale bars, 10 μm.) See also Movie S3 (control AB) and Movie S4 (Dgt4 AB). (D) Quantitation of the ratio between the EB1 intensity in the midzone and near the poles (see circles for zone of measurement). Mean and SEM are derived from three independent experiments (2–7 spindles measured for each time point in each independent experiment). Image published in: Petry S et al. (2011) Copyright © 2011. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |