Xenbase Image ID: 74490

Fig. 8. xnr5 and xnr6 can activate Smad2 and regulate their own expression before the MBT (A) 8-cell embryos were injected in one animal cell with fluorescent dextran alone (control) or with xnr1 (50 pg), xnr5 (20 pg), or xnr6 (20 pg) mRNAs. Whole mount ISH for xnr5, xnr6, and chd was performed on embryos fixed at the indicated stages. (B) Embryos from A were analyzed by qRT-PCR at stage 8 (preMBT). (C) 8-cell embryos were injected in one animal cell with GFP-Smad2 (200 pg) alone or with xnr5 mRNA (50 pg). Embryos were fixed at the 512-cell stage, processed for anti-GFP immunostaining, and sectioned for fluorescence imaging. DAPI was used to reveal nuclei. The graph shows the percent of DAPI positive nuclei that also contain GFP-Smad2. (D) Embryos injected as in C were fixed at various stages as indicated and stained for xnr6 expression. ui, uninjected. Image published in: Skirkanich J et al. (2011) Copyright © 2011. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue chrd.S chd, chordin, chrd.1, LOC108716939, X-chordin X. laevis Throughout NF stage 8 to NF stage 10   nodal5.2.L nodal5, nr5, xnr5, Xnr5-14 X. laevis Throughout NF stage 8 to NF stage 10   nodal6.L nodal6-a, nodal6-b, nr6, xnr6, xnr6b X. laevis Throughout NF stage 8 to NF stage 10   nodal6.L nodal6-a, nodal6-b, nr6, xnr6, xnr6b X. laevis Throughout NF stage 6.5 to NF stage 8   Image source: Published Larger Image Printer Friendly View

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