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Fig. 3. Histological analysis of Ptf1a and Ptf1a-VP16 transgenic whole guts. (A) Control whole gut section stained for amylase showing normal stomach (st), pancreas (p) and liver (l). (B) Single whole gut from TTR-Ptf1a:Elas-GFP transgenic tadpole was processed for histology after staining for amylase expression. 12-μm serial sections numbers correlate to specific section, with number 1 corresponding to the first section obtained from the isolated whole gut. The posterior small intestine (si) is evident in every section on the left side for orientation. (B) Section 5sophagus (e), pancreas (p) and liver (l) are all present. (C) Section 11he pancreas has almost completely replaced the stomach and duodenum (p + e). A small portion of remaining stomach tube is evident but contains no recognizable stomach cells and the tube is open. Part of the liver (l) can still be seen. (D) Section 18o gut tube is evident, and a large ectopic pancreas (p + e) is found in place of the stomach and duodenum. (E) Section 24he small intestine (si) first reappears, with pancreatic tissue and liver still present. We measured the lack of stomach and duodenum from sections 10 to 24 to be 168 μm in total. (F) Section from Ptf1a-VP16 transgenic whole gut stained with amylase. The ectopic pancreas (ep) is seen fused with the liver, and the normal pancreas adjacent but separate. Image published in: Jarikji ZH et al. (2007) Copyright © 2007. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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