Xenbase Image ID: 74582

Supplemental Figure 4 (Supports Figure 5). (A) Injection of 20 ng of HEB MO in both blastomeres at the 2-cell stage does not result in gross morphological defects at gastrulation (stage 10.5) or in early tailbud embryos (stage 22). (B) Co- injection of 10 ng of E2A MO with nuclear β-galactosidase mRNA in one blastomere at the 4-cell stage results in cell-autonomous inhibition of blastopore formation and of xbra expression. Red color represents red-gal substrate staining and marks the injected portion of the embryo. (C) Embryos co-injected with e2a MO and mouse e2a mRNA were compared to uninjected controls for expression of xbra (mesoderm) and sox17β (endoderm). While e2a MO injection led to downregulation of xbra, this was rescued by co-injection with mouse E2A mRNA. Image published in: Yoon SJ et al. (2011) Copyright © 2011. Image reproduced on with permission of the Publisher, Cold Spring Harbor Laboratory Press. This is an Open Access article. Gene Synonyms Species Stage(s) Tissue tbxt bra, brachyury, ntl, t, t-a, t-b, X-bra, Xbra, Xbrachyury X. tropicalis Throughout NF stage 10 mesoderm involuting marginal zone marginal zone sox17b.1 sox17, sox17-beta, Sox17beta, tSox17beta, Xsox17, Xsox17-beta X. tropicalis Throughout NF stage 10 endoderm vegetal endoderm Image source: Published Larger Image Printer Friendly View

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