Xenbase Image ID: 74594

Fig. 1. Cer-S, a secreted inhibitor of Nodal-related factors, inhibits formation of Spemann organizer. (A,B) cer-S mRNA (radial injection of 150 pg into each blastomere at the 4-cell stage) blocks dorsal lip formation. (C,D) gsc expression is blocked by cer-S, even after LiCl treatment that expands gsc expression to the entire mesoderm (insets). (E,F) δN-XTcf-3 mRNA (800 pg radially) inhibits goosecoid expression, and co-injection of Xnr1 (50 pg) restores it in the entire marginal zone. (G) RT-PCR analysis of Spemann organizer markers at stage 10. Lane 1: whole embryos. Lane 2: radial injection of cer-S (600 pg total) represses organizer genes. Lane 3: radial injection of Xnr1 mRNA (50 pg) upregulates organizer markers. Lane 4: the β-catenin pathway antagonist δN-XTcf-3 (800 pg) inhibits organizer markers, which are rescued by co- injection with 50 pg Xnr1 (lane 5). Xnr1 acts downstream of, or in parallel to, the β-catenin pathway. Siamois (Lemaire et al., 1995) is regulated by the β-catenin pathway (Brannon et al., 1997) independently of Xnr signals. Image published in: Agius E et al. (2000) Copyright © 2000. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue gsc.L goosecoid, gsc-a, gsc-b, Xgsc X. laevis Throughout NF stage 10.5 dorsal marginal zone Image source: Published Larger Image Printer Friendly View

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