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Fig. 2. Cer-S inhibits Xnrs but not activin, derrière, Vg1 and Xnr3. Co-injection of cer-S mRNA (150 pg into a single animal blastomere) inhibited ectopic blastopore lip formation by Xnr1 (50 pg, D, but not by activin (30 pg), derrière (150 pg) or A-Vg1 (50 pg) mRNA (AD. Xnr2 mRNA (150 pg) was also inhibited (data not shown). Although the doses used for each TGF-β mRNA differed, they all were titrated to elicit comparable biological responses. tALK4 (800 pg) mRNA blocked all TGF-β mesoderm inducers tested (AD. The ectopic blastopore lips are seen as a darker area in the injected animal cap region due to the apical constrictions of bottle cells (Lustig et al., 1996). (E) Xnr3 (1.2 ng) is a neural (NCAM) but not mesodermal (α-actin) inducer in microinjected animal caps (lanes 2 and 3). cer-S mRNA (600 pg) does not inhibit Xnr3 activity in animal caps (lanes 4 and 5). EF1α was used as a loading control. Image published in: Agius E et al. (2000) Copyright © 2000. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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