Xenbase Image ID: 74636

Fig. 2. Pre-MBT PGCs and somatic cells express CTD-P-Ser5, but not CTD-P-Ser2. Immunofluorescence of dissociated cells isolated from pre-MBT stage 8 embryos previously stained with DiOC6 (A) to identify PGCs. Monoclonal antibodies H5 and H14 were used to detect the expression of CTD phospho-serines 2 (B, P-Ser2) and 5 (D, P-Ser5), respectively, in PGCs (arrowheads) and somatic cells (arrows). Monoclonal antibody 8WG16 recognizes the unphosphorylated RNA Pol II large subunit (C, RNAPII) and served as a positive control. Image published in: Venkatarama T et al. (2010) Copyright © 2010. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue polr2a hrpb220, hsrpb1, LOC108703583, polr2, polra, rpbh1, rpiils, rpol2 Xenopus Throughout NF stage 13 somatic layer of lateral plate mesoderm Image source: Published Larger Image Printer Friendly View

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