Xenbase Image ID: 74639

Figure 4. Misexpression of Gem prevents neurogenesis. (A), Two-cell stage Xenopus embryos were injected in one cell as indicated (yellow) with beta-galactosidase mRNA coinjection for lineage tracing and analyzed for NCAM (A) or N-tubulin (DO) expression. (CT) C-terminal region. 3EA is an alanine sub- stitution mutant of acidic amino acids 18890 in Xenopus Gem (which correspond to mouse Gem amino acids 17476). Like GemE174Q (Fig. 2F,G), Xenopus Gem 3EA has severely reduced Brg1 binding (data not shown). Numbers indicate the number of affected embryos over the total number of embryos. In situ for markers is stained purple, and blue X-gal staining marks the injected side. Injected sides were oriented rightward. (P) Expression levels of Gem variants injected into Xenopus embryos. Embryos were injected as indicated at the two-cell stage and harvested at stage 12 to prepare lysates. Ten embryos were used for each sample, and one embryo-equivalent of lysate was loaded per lane for Western blotting with anti-Myc antibody. Image published in: Seo S et al. (2005) Copyright © 2005. Image reproduced on with permission of the Publisher, Cold Spring Harbor Laboratory Press. This is an Open Access article. Larger Image Printer Friendly View

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