Xenbase Image ID: 74640

igure 6. Loss of Gem activity results in premature neurogenesis. (A) Xenopus embryos were injected with 2.5 ng (A) or 1.5 ng (E,F) of GemMO or 20 ng of control MO (G,H) and probed as indicated (white). Red arrowheads in E and F mark the pos- terior end of N-tubulin expression in the medial stripe on each side of the embryo. (I) Reduction of Gem protein level in P19 cells by RNAi. (J) Reduction of Gem sensi- tized P19 cells to differentiate into neurons at a subthreshold level (50 ng) of NeuroD2. P19 cells were transfected as indicated and probed with TuJ1 antibody to detect neu- rons. GFP expression plasmid was cotrans- fected to trace transfected cells. Image published in: Seo S et al. (2005) Copyright © 2005. Image reproduced on with permission of the Publisher, Cold Spring Harbor Laboratory Press. This is an Open Access article. Larger Image Printer Friendly View

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