Xenbase Image ID: 74642

Fig. 4. Loss of Brg1 increases cell proliferation. (A-D) Whole-mount stage 14 embryos immunostained (brown) to detect phosphorylated histone H3 (PH3) after injection of standard MO (A) or xBrg1MO (B-D). (C,D) Lateral views of an xBrg1MO-injected embryo: (C) uninjected side; (D) xBrg1MO-injected side. (E) Transverse section of embryo in B, showing the injected side facing rightwards. Black arrowheads mark PH3-positive cells. n, notochord; s, somite. (F-H) Cell division was blocked by adding hydroxyurea (HU) from stage 12.5 until fixation at stage 15. (F) PH3-immunostained embryo showing cell cycle is efficiently blocked by HU treatment (xBrg1MO+β-galactosidase injection, blue; PH3 stain, brown). (G,H) xBrg1MO-injected embryos were raised in the presence of 30 mM HU from stage 12.5 and analyzed for Sox2 (G) and N-tubulin (H) expression. (I,J) Whole-mount TUNEL staining (brown) after injection of standard MO or xBrg1MO. A,B,F-J are dorsal views with injected side facing rightwards. Image published in: Seo S et al. (2005) Copyright © 2005. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue sox2.L anop3, LOC108718087, mcops3, Sox-2, XLSOX-2, Xsox-2, XSox2 X. laevis Throughout NF stage 18 neuroectoderm neural plate tubb2b.S N-Tub, n-tubulin, NBT, neural beta-tubulin, NST, ntubulin, Xn-tubulin X. laevis Throughout NF stage 18 neural tube Rohon-Beard neuron neural plate neural plate border Image source: Published Larger Image Printer Friendly View

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