Xenbase Image ID: 74662

Figure 4. BMP4 can induce intermediate neural plate tissue to form RB sensory neurons In situ hybridization of intermediate neural plate explants (A-D,F) or epidermal ectoderm (E) isolated at stage 11.5−12 and subsequently incubated with a heparin bead with or without hBMP4 (A-D,F) until stage-matched controls reached stage 24−25. (A) Intermediate explants incubated with a 0.5MBS-soaked bead never expressed the RB marker XHox11L2. Intermediate explants incubated with 1 ng/ml BMP4 stained positive for XHox11L2 (arrows in B) and XN-tubulin (arrows in C; white circle indicates position of the bead observed as an impression, since the bead was displaced during processing). (D) Expression of XSox2 in explants incubated with 1ng/ml BMP4 beads suggests that neural tissue is maintained in the area around the beads. (E) Epidermal explants stained positive for XKeratin, while neural plate explants incubated with 1 ng/ml BMP4 did not (F). Image published in: Rossi CC et al. (2008) Copyright © 2008. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue tlx3.S hox11l2, rnx, XHox11L2 X. laevis Sometime during NF stage 24 to NF stage 25 Rohon-Beard neuron tubb2b.S N-Tub, n-tubulin, NBT, neural beta-tubulin, NST, ntubulin, Xn-tubulin X. laevis Sometime during NF stage 24 to NF stage 25 Rohon-Beard neuron sox2.L anop3, LOC108718087, mcops3, Sox-2, XLSOX-2, Xsox-2, XSox2 X. laevis Sometime during NF stage 24 to NF stage 25 Rohon-Beard neuron krt12.4.L ck81, cytok, cytokeratin, E-keratin, epi-keratin, epidermal keratin, Epidermis-specific keratin, EpiK, epiker, EpK, epK, K81, xK81, xk81a, xk81a1 X. laevis Sometime during NF stage 24 to NF stage 25 non-neural ectoderm epidermis Image source: Published Larger Image Printer Friendly View

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