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Figure 6. Marker gene analyses of Insulin receptor substrate IRS-1-depleted embryos. A: IRS-1 knock down in neural tissue reduces the expression of the eye marker genes Rx1 and Pax6, but not the pan-neural marker gene Sox3 at stage 13. B: At stage 23, expression of Rx1, Pax6, and Otx2 in the eye is affected. In contrast, expression of Emx1, Krox20, and En2 in the brain is not affected. C: Coinjection of 0.5 ng of δ5′ untranslated region (UTR) IRS-1 RNA significantly reverted the IRS-1 morpholino oligonucleotide (MO) (20 ng) -induced reduction of Rx1 expression at stage 13. D,E: Quantitative representations are given. *P < 0.05, **P < 0.005, n, number of independent experiments; N, total number of embryos examined.Download figure to PowerPoint Image published in: Bugner V et al. (2011) Copyright © 2011. Image reproduced with permission of the Publisher, John Wiley & Sons. Gene Synonyms Species Stage(s) Tissue rax.S rax-a, rax-b, rax1, rx, rx-1, rx1, rx1a, Rx2A, Xrax, Xrx1, Xrx1A X. laevis Throughout NF stage 13 optic field pre-chordal neural plate rax.S rax-a, rax-b, rax1, rx, rx-1, rx1, rx1a, Rx2A, Xrax, Xrx1, Xrx1A X. laevis Throughout NF stage 23 eye retina emx1l.S Xemx1 X. laevis Throughout NF stage 23 forebrain telencephalon pax6.L an2, mgda, pax-6, pax6-a, pax6-b, wagr, XLPAX6, xpax6 X. laevis Throughout NF stage 13 lens placode spinal cord pre-chordal neural plate optic field pax6.L an2, mgda, pax-6, pax6-a, pax6-b, wagr, XLPAX6, xpax6 X. laevis Throughout NF stage 23 lens placode eye forebrain brain retina egr2.L EGR-2, Krox-20, krox20, XKr20, XKrox-20 X. laevis Throughout NF stage 23 rhombomere rhombomere R3 rhombomere R5 sox3.S Sry, Xlsox3, xSox3, Xtsox3 X. laevis Throughout NF stage 13 lens placode neural plate pre-chordal neural plate chordal neural plate otx2.S otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 X. laevis Throughout NF stage 23 eye brain forebrain midbrain Image source: Published Larger Image Printer Friendly View

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