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Figure 7. Insulin receptor substrate IRS-1 knock down affects the size of the lens placode. The width (w) and the height (h) of the the lens placodes visualized by FoxE3 staining were measured (represented by dotted lines), and ellipse area was calculated (π*w*h/4). The area of the lens placode on the IRS-1 morpholino oligonucleotide (MO) -injected side is significantly smaller compared with the uninjected side. In cases of Control MO treatment, no difference in the size of the lens placode of the injected versus uninjected side was observed. In total, 20 embryos from two independent experiments were analyzed. *P < 0.05, is, injected side; uis, uninjected side.Download figure to PowerPoint

Image published in: Bugner V et al. (2011)

Copyright © 2011. Image reproduced with permission of the Publisher, John Wiley & Sons.

foxe3.LXlens1, lens1, FoxE4X.laevisThroughout NF stage 26lens placode

Image source: Published

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