Xenbase Image ID: 74841

Figure 1. Localization of R9AP and syntaxin 3 in Xenopus photoreceptors. (A) Domain organization shared by R9AP and syntaxin 3. Both contain an N-terminal Habc domain, a SNARE homology domain, and a C-terminal transmembrane domain. (B) Immunofluorescence of R9AP (red) using the antibody against xR9AP2 in rod and cone outer segments of adult Xenopus retina. (C) Immunostaining of syntaxin 3 (green) in adult Xenopus retina. (B and C) The nuclei are counterstained with Hoechsts (blue). RPE, retinal pigmented epithelium; OS, outer segment; IS, inner segment; ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GC, ganglion cell. No signals were revealed in controls where the primary antibodies were omitted. (D) Immunostaining of syntaxin 3 (green) in two examples of broken rod outer/inner segment preparations double labeled either with phalloidin (red; top), which labels the actin-rich calycal processes (CP), or with acetylated α-tubulin (red; bottom), which labels the axoneme within the connecting cilium and the outer segment. The right panels are the merged image with the shape of the entire cell fragment outlined in white, as seen in the transmitted light channel. Note that multiple calycal processes are seen as the image is a z-stack projection. (E) Cartoon of the rod photoreceptor with the regions containing R9AP in red and the regions containing syntaxin 3 outlined in green. Image published in: Baker SA et al. (2008) © 2008 Baker et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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