Xenbase Image ID: 74848

Figure 8. Localization of ER and mitochondrial-targeted GFP-tagged transmembrane domains and their length mutants in transgenic Xenopus rods. The following constructs were expressed as GFP fusion proteins in Xenopus rods (see Table S2 for sequence details, available at http://www.jcb.org/cgi/content/full/jcb.200806009/DC1): (A) transmembrane domain from microsomal cytochrome b5 (Cyt b5); (B) transmembrane domain from cytochrome b5 elongated by four amino acid residues; (C) transmembrane domain from Bcl XL; (D) transmembrane domain from Bcl XL bearing a point mutation (K233S); (E) transmembrane domain from Bcl XL bearing the K233S mutation and elongated by four amino acid residues. OS, outer segment; IS, inner segment; N, outer nuclear layer; ST, synaptic terminal. Bars, 5 μm. Image published in: Baker SA et al. (2008) © 2008 Baker et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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