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Fig. 5. δXTcf3 expands the neuronal precursor pool but inhibits neuronal differentiation. (A) mRNA encoding δXTcf3 or δXLef1 was coinjected with β-gal into one blastomere at the two-cell stage. Embryos were examined by in situ hybridization at stage 18 for expression of Sox3 (i, ii); ESR7 (iii, iv); Xash (v, vi); and Delta (vii, viii). All embryos are viewed dorsally with anterior down. Blue staining represents β-gal staining and arrows indicate injected side. δXTcf3 expands expression of Sox3 (ii), and ESR7 (iv), but inhibits markers of neuronal differentiation including Xash (vi) and Delta (viii). While δXLef1-injected embryos show a limited expansion of markers of neuronal precursors, there is no significant effect on markers of neuronal differentiation. (B) Embryos were injected with mRNA encoding β-gal and δXTcf3, Neurogenin, or both. Arrows indicate injected side. (i) δXTcf3 inhibits N-tubulin. Embryos injected with mRNA encoding Neurogenin induces ectopic neural differentiation marked by N-tubulin. (iii) δXTcf3 is able to block ectopic neural differentiation caused by Neurogenin. Image published in: Heeg-Truesdell E and LaBonne C (2006) Copyright © 2006. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue sox3.S Sry, Xlsox3, xSox3, Xtsox3 X. laevis Throughout NF stage 18 neuroectoderm neural plate hes5.2.L bHLHb38, esr-7, esr3, esr7, hes5.2-a, hes5.2-b X. laevis Throughout NF stage 18 neural fold dll1 delta-1, Delta-1, delta1, x-delta, X-delta-1, Xdelta-1, Xdelta1, XDelta1 X. tropicalis Throughout NF stage 18 neural crest anterior neural fold anterior neural ridge neural tube Image source: Published Larger Image Printer Friendly View

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