Xenbase Image ID: 74862

Fig. 6. Inhibition of Canonical Wnt signals induces neural tissue. (A) Schematic showing the XLef1 and XTcf3 constructs utilized in these experiments compared to full-length coding regions. (B) Embryos were injected in one blastomere at the two-cell stage with mRNA encoding constitutively repressing forms of XLef1 or XTcf3 (XLef1HMGEnR i, vi; XTcf3HMGEnR, ii, vii); the β-catenin binding domain of XLef1 or XTcf3 (NLEF, iii, viii; NTCF, iv, ix); or were injected in one blastomere at the eight-cell stage with morpholinos targeting β-catenin (v, x). For all means of inhibiting canonical Wnt signals, expression of the neural crest marker Slug was inhibited (i) and expression of the pan-neuronal marker Sox3 was dramatically expanded (vi). (C) When expressed at the same level, δXTcf3 and δXLef1 Tailswitch have equivalent ability to inhibit Slug (iv). However, δXTcf3 expands Sox3 expression (iii, iv) more potently than δXLef1 Tailswitch (vii, viii). Image published in: Heeg-Truesdell E and LaBonne C (2006) Copyright © 2006. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue snai2.L slug, snai2-a, snai2-b, Snail2, xSlu, xslug, XSnail2 X. laevis Throughout NF stage 16 neural crest sox3.S Sry, Xlsox3, xSox3, Xtsox3 X. laevis Throughout NF stage 15 pre-chordal neural plate neuroectoderm neural plate chordal neural plate Image source: Published Larger Image Printer Friendly View

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