Xenbase Image ID: 74904

Figure 7. Requirement for exogenous Ago2 in pre-miR-451 processing. (A) Schematic representation of the evolutionarily conserved short hairpin structure of vertebrate pre-miR-451, indicating the mature miRNA (bold line) and the initial Ago2 cleavage site at nucleotide 30. (B) 32P-labeled pre-miR-451 RNA was injected into oocytes (left) or one- to two-cell embryos (right) that were untreated (control) or programmed to express the indicated hAgo2 proteins, and pre-miR-451 processing was monitored by denaturing PAGE. Embryo stages 10.5 and 12 correspond to ∼10 and ∼12.5 h post-fertilization. The identity of the processing products was confirmed by RNase T1 fingerprinting (not shown). Image published in: Lund E et al. (2011) Copyright © 2011. Image reproduced on with permission of the Publisher, Cold Spring Harbor Laboratory Press. This is an Open Access article. Larger Image Printer Friendly View

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