Xenbase Image ID: 75016

Fig. 5. Whole mount in situ hybridization analysis of VEGF expression. Embryos in A, B, E, and F were photographed at 70X, C at 30X, and D at 35X. A: Lateral view of a stage 21 embryo showing regions of staining in the aortic arches (out of the plane of focus; arrowhead), in the pharyngeal endoderm (short arrow), in the floor of the neural tube above the tip of the notochord (open arrowhead), and in the archencephalon (long arrow). B: Dorsal view of embryo shown in A. The region of neural staining is located at the midline of the embryo between the eye anlagen (large arrow). Aortic arch staining is indicated by small arrowheads. C: Lateral view of stage 26 embryo. VEGF expression can be seen in the cephalic regions described above, and in the nuclei of the somites (arrowheads) and of the hypochord (large arrow). D: Lateral view of stage 34 embryo. VEGF expression has resolved to sharper domains in the aortic arches (thin arrow) and additional expression can be seen in the developing glomus (open arrowhead). Expression continues in the somites (arrowheads). Note that expression of VEGF in the hypochord (large arrow) is declining in the anterior portion of the embryo. E: Higher magnifica- tion, lateral view of embryo in D showing nuclear staining in the hypochord (large arrow), in the somites (arrowheads), and staining in the glomus (open arrowhead). F: Dorsal view of the somite expression (arrowheads) and of the hypochord nuclei at the midline of the embryo (arrow). Image published in: Cleaver O et al. (1997) Copyright © 1997. Image reproduced with permission of the Publisher, John Wiley & Sons. Gene Synonyms Species Stage(s) Tissue vegfa.L vegf, vegf-a, vegfa-a, vegfa-b X. laevis Throughout NF stage 21 blood vessel aortic arch circulatory system pharyngeal epithelium endoderm neural tube floor plate anterior neural tube vegfa.L vegf, vegf-a, vegfa-a, vegfa-b X. laevis Throughout NF stage 26 blood vessel aortic arch intersomitic vessel anterior neural tube hypochord vegfa.L vegf, vegf-a, vegfa-a, vegfa-b X. laevis Throughout NF stage 33 and 34 glomus somite hypochord aortic arch Image source: Published Larger Image Printer Friendly View

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