Xenbase Image ID: 75039

Fig. 4. Physical interactions between Crescent and Wnts as assayed by coimmunoprecipitation. Experimental procedures are described in Materials and Methods. (A) and (B) Crescent interacts with Wnts that transduce non-canonical Wnt signaling (Xwnt11, Xwnt5a, and mouse Wnt4) as well as those that transduce canonical signaling (Xwnt8 and Xwnt3a). Xwnt11-Myc, Xwnt5a-Myc, or Xwnt8-Myc (A) or Xwnt8-Myc, Xwnt3a-Myc, or mouse Wnt4-Myc (B) was coimmunoprecipitated with either Cres-F (A) and (B) or Frzb1-F (A). As a positive control, Xwnt11-Myc was coimmunoprecipitated with the FLAG-tagged ectodomain of Xfz7 (Exfz7-F). (C) Cres-FZδ1-F forms a complex with Xwnt11-Myc. (D) Comparison of complex formation between Crescent and Frzb1 for Xwnt11, Xwnt5a, and Xwnt8. Cres-F shows a higher activity to form a complex with Xwnt11-Myc and Xwnt5a-Myc than does Frzb1-F, whereas Cres-F and Frzb1-F show similar activities for Xwnt8-Myc. Numbers in the panels indicate the amounts of cres-F or frzb1-F mRNA (ng/embryo) injected. Note that frzb1-F mRNA was injected at higher doses than cres-F mRNA to immunoprecipitate equivalent amounts of proteins. The horizontal axes indicate the amounts of Cres-F or Frzb1-F protein immunoprecipitated with anti-FLAG antibody, in arbitrary units. The vertical axes indicate the amounts of Myc-tagged Wnt proteins coimmunoprecipitated with either Cres-F (red line) or Frzb1-F (blue line), in arbitrary units. The amount of Wnt mRNAs injected was fixed at 500 pg/embryo in all experiments. Image published in: Shibata M et al. (2005) Copyright © 2005. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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