Xenbase Image ID: 75041

Fig. 6. Crescent antagonizes the action of Xwnt11 on CEMs in the neural plate, but enhances it in the dorsal mesoderm. (A) and (B) Coexpression of Crescent and Xwnt11 cancels out their action on CEMs in the neural plate. (A) Morphological appearance. Four-cell-stage embryos were injected in the dorsal animal pole region with mRNAs, as indicated, together with nβ-gal mRNA. Embryos at the tailbud stage (stage 28) are shown. (B) Summary of the phenotypes shown in (A). (C) and (D) Dorsal flexure elicited by Crescent or Xwnt11 is enhanced by coexpression of these proteins in the dorsal mesoderm. (C) Morphological appearance. Four-cell-stage embryos were injected with mRNA, as indicated, in the dorsal equatorial region. Embryos at the tailbud stages (stages 33–34) are shown. (D) Summary of the phenotypes shown in (C). The extent of dorsal flexure elicited by dorsal animal pole or equatorial injection was classified by blind scoring as follows: −, normal; +, weak; ++ moderate; +++, severe; s.a, short axis without dorsal flexure. Amounts of injected mRNAs (pg/embryo): β-globin, 100 (A), 600 (C); crescent, 100 (A), 50 (C); Xwnt11, 500 (A), 20 (C). Image published in: Shibata M et al. (2005) Copyright © 2005. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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