Xenbase Image ID: 75042

Fig. 7. Effects of Crescent on the activity of Xwnt11 in neuralized and mesodermalized animal caps. (A) and (B) Crescent and Xwnt11 antagonize each other in the inhibition of XBF2-induced elongation of animal caps. (A) Morphological appearance of animal caps. (B) Summary of the animal cap elongation assays shown in (A). (C) and (D) The inhibitory action on activin-induced elongation of animal caps by Crescent and Xwnt11 is enhanced when the proteins are coexpressed. (C) Morphological appearance of animal caps. (D) Summary of animal cap elongation assays shown in (C). See Fig. 2 for the experimental procedures. The extent of animal cap elongation induced by activin treatment or overexpression of XBF2 was classified by blind scoring as follows: −, no elongation; +, weak elongation; ++ moderate elongation; +++, strong elongation. Amounts of injected mRNAs (pg/embryo): β-globin, 600 (A), 100 (C); crescent, 20 (A), 5 (C); Xwnt11, 500 (A), 100 (C); XBF2, 250. Image published in: Shibata M et al. (2005) Copyright © 2005. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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