Xenbase Image ID: 75043

Fig. 8. (A) Physical interactions of Cres-F and Xwnt11-Myc are similar in activin-treated and XBF2-expressing animal caps. Coimmunoprecipitation assays with Xwnt11-Myc and Cres-F were performed using activin-treated and XBF2-expressing animal caps. (B) Hyperphosphorylation of Dsh by Xwnt11 is downregulated by Crescent in both activin-treated and XBF2-expressing animal caps (upper band). (C) and (D) Inhibition of animal cap elongation by Crescent was rescued by coexpression of Cdc42T17N, a dominant-negative construct of Cdc42, in the activin-treated, but not in XBF2-expressing caps. (D) Summary of animal cap elongation assays. The extent of animal cap elongation induced by activin treatment or XBF2 overexpression was classified by blind scoring as follows: −, no elongation; +, weak elongation; ++ moderate elongation; +++, strong elongation. Amounts of injected mRNAs (pg/embryo): β-globin, 250 (A), 2,000 (B); Xwnt11-Myc, 500; Xwnt11, 1000 (B), 500 (D); cres-F, 250; crescent, 500 (B), 10 (C), 50 or 10 (D); Myc-Dsh, 250; XBF2, 250; Xror2, 200; Cdc42T17N, 600. Image published in: Shibata M et al. (2005) Copyright © 2005. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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