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Fig. 4. The effects of XHR1-VP16-GR on the expression of possible target genes and neuronal markers. (A) ESR1, ESR9, XLCL2, Xdelta1, Xngnr1, and XHR1 are activated by XHR1-VP16-GR in the presence of CHX. Embryos were injected into the dorsal left blastomere at the four-cell stage with XHR1-VP16-GR mRNA (100 pg), and treated with or without CHX for 30 min before the addition (or not) of DEX at the gastrula stage (stage 11), as indicated. The expression of ESR1 (a), ESR9 (b), XLCL2 (c), Xdelta1 (d), Xngnr1 (e), and XHR1 (f) were analyzed at stage13 by WISH. (B) The effects of XHR1-VP16-GR on the expression of neuronal markers. The expression of Xlim3 (a), Xash3 (b), and β2-tubulin (c) was analyzed at stage 13 by WISH. Fractions indicate the proportion of the presented phenotype per total number: numbers in black, normal expression; numbers in red, upregulation; numbers in green, disrupted expression; numbers in blue, downregulation. Black arrows in Ad,e, pre-MHB region; red arrow in Ba, ectopic expression; black arrow in Bb, reduced expression. Image published in: Takada H et al. (2005) Copyright © 2005. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue hes5.L esr-1, esr1, hes5.1, XESR-1 X. laevis Throughout NF stage 13 central nervous system neural plate neuroectoderm neural fold Rohon-Beard neuron non-neural ectoderm circumblastoporal collar mesoderm anterior neural fold hes5.6.L X. laevis Throughout NF stage 13 central nervous system neural plate neuroectoderm non-neural ectoderm neural fold circumblastoporal collar mesoderm Rohon-Beard neuron anterior neural fold neural plate border lbh.L xlcl2 X. laevis Throughout NF stage 13 midbrain anterior neural plate pre-chordal neural plate dll1.L delta-1, Delta-1, delta1, x-delta, X-delta-1, Xdelta-1, Xdelta1, XDelta1 X. laevis Throughout NF stage 13 midbrain hindbrain neural plate neuroectoderm neural plate border anterior placodal area neurog2.L atoh4, neurog2-a, neurog2-b, neurogenin 2, neurogenin-2, neurogenin-related-1, neurogenin2, ngn-2, ngn2, ngnr, ngnr 1a, Ngnr-1, Ngnr1, X-ngnr-1, xngn2, Xngnr-1, XNgnr1 X. laevis Throughout NF stage 13 neural plate neuroectoderm presumptive midbrain-hindbrain boundary non-neural ectoderm hes7.1.L HES-related 1, XHR1 X. laevis Throughout NF stage 13 midbrain hindbrain midbrain-hindbrain boundary neuroectoderm lhx3.L LIM-3, lim3, xLIM-3 X. laevis Throughout NF stage 13 neural plate neuroectoderm neural groove ascl2.S ascl2-a, ascl2-b, ash2, ash3, ASH3b, bhlha45, xash-3, xash2, xash3 X. laevis Throughout NF stage 13 neural plate neuroectoderm neural fold Image source: Published Larger Image Printer Friendly View

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