Xenbase Image ID: 75049

Fig. 5. The effects of gain-of-function or loss-of-function of XHR1 on expression gene. (A) Misexpression of XHR1 represses ESR1 and XLCL2 expression. XHR1 mRNA (12.5–25 pg) was injected into the dorsal left blastomere at the four-cell stage. ESR1 (a) and XLCL2 (b) expression was examined by WISH at stage 13–14. Each stripe in which primary neurons arise is numbered in the expression of ESR1. 1, Medial stripe; 2, intermediate stripe; 3, lateral stripe. Arrows, reduced expression. (B) Effects of XHR1-MOs on the expression of ESR1 (a–d), ESR9 (e, f), Xdelta1 (g, h), and Pax2 (i–l). XHR1-MOs (a, e, g, i) or XHR1-5mmMO (b, f, h, j) was injected at the four-cell stage, and globin (c, k; negative control) or XHR1 (d, l) mRNA was injected with nβ-gal mRNA at the eight-cell stage. (a′, e′) Magnification of the same embryo as shown in panels a and e. Black arrows, reduced expression; red arrows, ectopic expression. Fractions in black, normal expression; in blue, downregulation; in red, upregulation. Injected doses: MOs, 6 or 3 (i, j) ng; globin or XHR1 mRNA, 3–10 (d), or 0.3–1 (l) pg. Image published in: Takada H et al. (2005) Copyright © 2005. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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