Xenbase Image ID: 75072

Fig. 1.(A-D) αT4 globin expression in mid to late tailbud stage embryos and expression patterns of GATA-2 in mid-neurula stages. (A,B) Albino embryos probed for αT4 globin mRNA by whole-mount in situ hybridisation at stage 35 (A) and stages 24-26 (B). The foregut staining at the rostral (left) end of the mid tail bud embryos (B) is non-specific. The V- shape of the ventral blood islands at mid-tailbud (B) extends caudally as the embryo matures (A). (C-E) In situ hybridisations of mid-neural fold (stage 15) non- albino embryos hybridised with an antisense GATA- 2 probe. (C) Low power magnification showing strong ventral (v) and lateral but not dorsal (d) labelling. Arrowheads define the boundary between the GATA-2 positive and GATA-2 negative regions. (D) Higher power magnification showing strong peripheral labelling in the sensorial (inner) ectoderm (arrow) and weaker labelling in the mesoderm (double arrows) and endodermal cells (arrowhead). [NOTE shown as separte image XB-IMG- ](E) Stage 15 non-albino embryos probed for GATA-2 RNA (purple) by whole-mount in situ hybridisation, before clearing. Staining is apparent throughout the ventral surface up to the edges (dashed line) of the developing neural plate (np, brown due to pigmentation) giving rise to a ennis ballpattern. The embryo in the centre (V) is viewed from the ventral aspect. Image published in: Walmsley ME et al. (1994) Copyright © 1994. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue hba4.S alphaT4-globin, aT4 Globin, T4 globin X. laevis Sometime during NF stage 26 to NF stage 32 anterior ventral blood island ventral blood island Image source: Published Larger Image Printer Friendly View

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