XB-IMG-75092
Xenbase Image ID: 75092
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Fig. 1. Comparison of the phenotype penetrance between the redundant set and the unique full-length set screen. One hundred and six pools of a redundant neurula stage cDNA library (pool size 96 clones=10.176 cDNAs) and 120 pools of the unique full-length (FL) gastrula/neurula cDNA library (pool size eight clones=960 unique and full-length cDNAs) were screened by overexpressing ≈5 ng of in vitro transcribed RNA in one cell of two-cell stage embryos. Embryos were analysed at neurula and tadpole stages for changes in morphology and in the neural differentiation marker N-tubulin. Only phenotypes with a penetrance (percentage of affected embryos in a batch) of 40% or more were scored as ‘real’ but analysis for lower penetrance is also shown. In the redundant set screen ≈10% (n=11) of the screened pools showed a phenotype (≥40% penetrance) whereas this was raised to 50% (n=60) in the FL set screen (green star). At a higher penetrance (≥80%) there is also a big difference in phenotype recovery between the redundant and FL set screen (3 vs. 28%). Thus the FL set screen was five-fold more sensitive in detecting gain-of-function phenotypes compared to an unmodified cDNA library. Image published in: Voigt J et al. (2005) Copyright © 2005. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |