Xenbase Image ID: 75093

Fig. 2. Phenotypic categories found when screening the unique FL set pools. Sixty pools of the unique full-length gastrula/neurula stage cDNA library gave rise to an overexpression phenotype (at least 40% penetrance). Nine major categories (x-axis) of phenotypes, for which representative images are shown below each bar, were observed and scored. Phenotypes were classified as secondary axes (rather than ectopic tissue outgrowth) if the ectopic tissue shows axial organisation, like secondary heads (at tadpole stage) or complete secondary neural tubes (at neurula stage). Ectopic tissue outgrowth comprises tumour-like growths, protrusions (which lack clear axial structures) and other abnormal thickenings observed. Blisters are oedema-like appearances, which do not contain tissue. Phenotypes such as loss/shedding of cells and or limited cell death were grouped into tissue loss. In contrast, embryos that showed global death are classified in death. Since most of the phenotypes observed were complex, many of the pools were included in more than one category (thus the sum of the percentages is greater than 100%). Of particular interest were phenotypes that affected the neural differentiation marker N-tubulin (stars), since they might contain an activity involved in the neurogenesis pathway. (Purple staining in images: N-tubulin, light blue staining: lineage tracer LacZ). Image published in: Voigt J et al. (2005) Copyright © 2005. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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