Xenbase Image ID: 75094

Fig. 5. Additional detail on the overexpression of 4 selected genes and Rab32 expression. (a) XER 81 RNA injections expand X-Sox3 (purple) on the injected side. (b+c) Frizzled10B RNA injections enhance the medial (motorneuron) stripe of N-tubulin expression (white arrowhead, in wholemount (B) and transverse (C) view), in addition to the ectopic expression of N-tubulin observed in the floorplate (shown in Fig. 4b). (dashed line-midline, l-lateral, i-intermediate, m-medial). (d+e) 1 μm sections of Rab32 (1 ng) injected (E) and control Xenopus laevis (D) embryos. (D) In wild type embryos the melanosomes (darkly pigmented vesicles, arrow) are localised predominantly under the apical surface, but some can be found deeper within the cells (arrowhead). In contrast, when Rab32 was overexpressed the melanosomes were clustered tightly together and localised under the apical surface (arrows). (f) Transverse section (30 μm) of a bleached Xenopus tropicalis tailbud embryo in situ hybridised with an antisense probe to Rab32. Rab 32 is expressed in the retinal pigment epithelium (purple). Image published in: Voigt J et al. (2005) Copyright © 2005. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue sox3 Sry, Xlsox3, xSox3, Xtsox3 X. tropicalis Throughout NF stage 17 neural plate tubb2b N-Tub, n-tubulin, NBT, neural beta-tubulin, NST, ntubulin, Xn-tubulin X. tropicalis Throughout NF stage 17 neuron Rohon-Beard neuron rab32 X. tropicalis Throughout NF stage 26 eye retinal pigmented epithelium Image source: Published Larger Image Printer Friendly View

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