Xenbase Image ID: 75185

FIG. 3. The Pax-6 promoter drives the expression of GFP in a similar domain to that of endogenous Pax-6. Panels A, D, G, and J show images of embryos stained for endogenous Pax-6 RNA by whole-mount in situ hybridization at stages 14, 25, 30, and 32, respectively. Panels B, E, H, and K show images of Pax-6GFP transgenic embryos stained for GFP RNA by whole-mount in situ hybridization at stages 14, 25, 30, and 32, respectively. At stage 14, both endogenous Pax-6 (A) and GFP transgene (B) are expressed in a broad arc at the anterior neural plate, which gives rise to the forebrain and eyes. Expression is also apparent in two lateral stripes in the posterior neural plate that will give rise to the hindbrain and neural tube. (C) A stage 14 transgenic embryo stained for endogenous Pax-6 RNA (magenta) and GFP RNA (light blue). (I) A stage 32 half transgenic embryo stained by whole-mount in situ hybridization to Pax-6 (light blue) and GFP (magenta). Note strong expression of GFP transgene in the telencephalon (arrow). (F), (L) Fluorescence images in Pax-6GFP transgenic embryos at stages 25 and 32, respectively. Image published in: Hartley KO et al. (2001) Copyright © 2001. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue pax6.L an2, mgda, pax-6, pax6-a, pax6-b, wagr, XLPAX6, xpax6 X. laevis Throughout NF stage 14 pre-chordal neural plate neural tube pax6.L an2, mgda, pax-6, pax6-a, pax6-b, wagr, XLPAX6, xpax6 X. laevis Sometime during NF stage 25 to NF stage 29 and 30 eye hindbrain optic vesicle pax6.L an2, mgda, pax-6, pax6-a, pax6-b, wagr, XLPAX6, xpax6 X. laevis Throughout NF stage 32 eye midbrain hindbrain spinal cord trigeminal placode Image source: Published Larger Image Printer Friendly View

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