Xenbase Image ID: 75202

Fig. 3. Misexpression of XBF-1 and qin suppresses endogenous and induces ectopic primary neurogenesis. Embryos were injected with XBF-1/lacZ (A-G,K), qin/lacZ (H-J) RNA, or were uninjected (L) and were processed for X-gal staining (light blue) and whole-mount in situ hybridisation for N-tubulin (purple). Dorsal (A,C,I,L) and side (B,D,E,J,K) views are shown, anterior to the right. Black arrowheads connect dorsal and lateral views of the same embryos. The lateral views show that the ectopic N-tubulin forms far from the dorsal midline at the lateral and even ventral side of the embryo, outside the XBF-1/lacZ-expressing ectoderm but at the boundary of the high expressing ectoderm. Similarly, XBF-1/lacZ injection of one blastomere of a 32 cell stage embryo (K), produces ectopic N-tubulin (arrow in K) at the boundary of the high XBF- 1/lacZ-expressing patch. (F) The XBF-1-injected side and (G) the uninjected control side of a tadpole stage embryo. In E and F, note that the ectopic N-tubulin stripe follows the boundary of X-gal staining and has formed perpendicular, rather than the normal parallel, to the antero- posterior axis. (H) A high magnification view of the lateral side of an embryo similar to the one shown in (D); it shows that there is no overlap between the high XBF-1/lacZ-expressing cells (blue) and the N-tubulin-expressing cells (brown/purple). The X-gal staining is nuclear while the in situ signal is cytoplasmic. In L a white broken line indicates the dorsal midline of the neural plate and separates the three bilaterally symmetrical stripes of N-tubulin expression. a, anterior; p, posterior. Image published in: Bourguignon C et al. (1998) Copyright © 1998. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

Return to previous page