Xenbase Image ID: 75205

Fig. 6. High XBF-1-expressing cells do not overlap with N-tubulin-positive cells. (A-H) Transverse sections through embryos injected with high and low concentrations of XBF-1/lacZ of XBF-1-myc as indicated. Staining for X-gal (light blue) and myc (orange) is nuclear while the in situ signal for N-tubulin (purple) is cytoplasmic. In all panels a solid line passes through the notochord and indicates the dorsal midline. (B,D,F,H) High magnification views of the lateroventral side of the embryo, therefore N-tubulin shown in these frames is ectopic. At the high XBF-1 concentration (B), broken lines delimit a cluster of ectopic N- tubulin cells formed adjacent to the ectoderm that shows detectable X-gal staining. At high XBF-1 concentration (A,B,E,F) N-tubulin cells do not express detectable lacZ or myc. By contrast at low XBF-1 concentration (C,D,G,H) some cells that express ectopic N-tubulin have detectable levels of nuclear X-gal or myc staining (arrows) while others do not (arrowhead). See text for details. Note that ectopic N-tubulin forms in the deep layer of the ectoderm, where the endogenous N-tubulin is also located. Image published in: Bourguignon C et al. (1998) Copyright © 1998. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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