XB-IMG-75207
Xenbase Image ID: 75207
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Fig. 8. Lateral inhibition is activated by XBF-1 and qin and contributes to the dispersed pattern of ectopic N-tubulin expression. (A) Embryos were injected with 0.5 ng qin or XBF-1 RNA, co- injected with lacZ RNA and assayed for X-gal staining (light blue) and X-Delta-1 expression (purple). In the qin-injected embryo, the injected side (shown on the left) expresses ectopic X-Delta-1 (arrow) while the control side (shown on the right) does not. In the XBF-1- injected embryo, the left panel represents a ventral view and the right panel a section through the ventral side of the embryo under high magnification, both showing ectopic X-Delta-1 expression in purple. (B) Embryos were injected with qin, XBF-1, qin/X-Delta-1 stu (a dominant negative form of X-Delta-1), XBF-1/X-Delta-1 stu or X- Delta-1 stu RNA, co-injected with lacZ RNA and assayed for X-gal staining (light blue) and N-tubulin expression (purple). In the qin- and XBF-1-injected embryos, the pattern of ectopic neuronal differentiation is less dispersed in the presence of X-Delta-1 stu suggesting that lateral inhibition limits the number of cells that adopt a neuronal fate in response to qin or XBF-1. However, neuronal differentiation is not observed in areas that express high levels of qin (identified by strong uniform X-gal staining, middle panels), even in the presence of X-Delta-1 stu. A white dotted line indicates the dorsal midline, for comparison between the injected and uninjected side. Note that both the high dose uppression of endogenous-ectopic inductionphenotype (top two frames, middle panel) and the low dose expansion of endogenousN-tubulin phenotype (lower frame, middle panel) are affected by co-expression of X-Delta-1stu. Image published in: Bourguignon C et al. (1998) Copyright © 1998. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |