Xenbase Image ID: 75209

Fig. 10. Misexpression of XBF-1-E1A versus XBF-1-EnR and XBF-1 has opposite effects on anterior development. Embryos were injected with various RNAs as indicated on the side of each set of panels and were analysed by double in situ hybridisation for N-tubulin (magenta) and XOtx2 (light blue), or by in situ hybridisation for XOtx2 (purple) and X-gal staining (light blue), as indicated. Dorsal (left panels) and anterior (middle and right panels; injected side to the left) views are shown. XBF-1-EnR reduces XOtx2 expression while XBF-1-E1A expands XOtx2 expression locally. XBF-1 also reduces XOtx2 expression (bottom right). The XBF-1-injected embryo shown in the middle panel has normal XOtx2 expression, presumably because it did not receive RNA anteriorly. Some XBF-1- EnR embryos show ectopic N-tubulin anteriorly, in the area where XOtx2 is suppressed (second row from top). Image published in: Bourguignon C et al. (1998) Copyright © 1998. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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