Xenbase Image ID: 75219

Fig. 8. RA applied at blastula stages but not at late gastrula stages, posteriorises the anterior neural plate and simultaneously induces premature anterior N-tubulin expression. Xenopus embryos were either left untreated (A,D) or treated with RA when sibling embryos reached stage 9 (B,E) or stage 12. 5 (C,F), and analysed simultaneously for the expression of Xotx2 and N-tubulin. Xotx2 staining is light blue while N-tubulin staining is magenta in color. Embryos shown in A-C have been cleared in benzyl benzoate in order to reveal possible N-tubulin staining in the deep regions of the neural tissue and are shown in D-F, respectively. Uninjected embryos expresses Xotx2 but no N-tubulin in the anterior neural plate (A,D). In embryos that have been treated with RA at stage 9, the anterior markers Xotx2 is suppressed and N-tubulin is massively induced in the anterior end of the neural ectoderm (B,E). In embryos that have been treated with RA at stage 12.5 the anterior marker Xotx2 is suppressed to a lesser degree than in embryos treated with RA at stage 9 (C,F). When cleared, these embryos show few N- tubulin cells anteriorly (F). Image published in: Papalopulu N and Kintner C (1996) Copyright © 1996. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue tubb2b.S N-Tub, n-tubulin, NBT, neural beta-tubulin, NST, ntubulin, Xn-tubulin X. laevis Throughout NF stage 17 trigeminal placode neural fold neural tube forebrain otx2.S otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 X. laevis Throughout NF stage 17 optic field eye primordium pre-chordal neural plate Image source: Published Larger Image Printer Friendly View

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