Xenbase Image ID: 75241

Figure 3. Crossing a recovered A7 F1 male with the C5 reporter strain. Larvae obtained from this crossing were selected for blue fluorescence, indicating the presence of the reporter gene LCMV:ECFP(loxP)(FRT)EYFP. A: Lateral view and ventral view of the head of a Cre-positive tadpole in the blue filter set. B: The same tadpole in the yellow filter set shows yellow fluorescence in tail myotomes and myofibrils of the jaws. C: Lateral view and ventral view of the head of a Cre-negative tadpole in the blue filter set. D: The same tadpole in the yellow filter set displays fluorescence that represents the bleed-through of the CMV driven ECFP activity. E: A Cre-positive tadpole at a later stage, ventral view. Left panel: blue filter set. Right panel: yellow filter set. No yellow fluorescence was observed in the heart. F: A Cre-positive tadpole, close up from the head, dorsal view. Left panel: blue filter set. Right panel: yellow filter set. Myofibrils near the eye show activation of the EYFP reporter gene. Scale bars = 1 mm.Download figure to PowerPoint Image published in: Waldner C et al. (2006) Copyright © 2006. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View

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