Xenbase Image ID: 75242

Figure 4. Crossing a recovered A7 F1 female with the G/R reporter strain. Larvae obtained from this crossing were selected for green fluorescence, indicating the presence of the reporter gene CAG:EGFP(loxP)DsRed2. A: Lateral view of a Cre-positive tadpole in the green filter set (upper panel). The same tadpole in the red filter set shows red fluorescence in tail myotomes (lower panel). Unspecific activation of red fluorescence was also observed (arrows). B: A Cre-negative tadpole showing mosaic expression of the CAG promoter driven EGFP gene (upper panel) and no red fluorescence in the red filter set (lower panel). C,D: Examples for induction of red fluorescence as well as induction of green fluorescence in muscle cells at a later developmental stage. C: Cre-positive larvae showing activation of green fluorescence in tail myotomes (upper panel, left) as well as activation of the red fluorescent reporter gene (lower panel, left). The right-hand panels show close-ups from myofibrils of the jaws in the respective filter sets. D: A Cre-negative sibling showing background green fluorescence in tail myotomes (upper panel) and lack of red fluorescence (lower panel). Scale bars = 1 mm.Download figure to PowerPoint Image published in: Waldner C et al. (2006) Copyright © 2006. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View

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