Xenbase Image ID: 75243

Figure 5. Crossing a recovered A7 F1 male with the GPL8 reporter strain. Larvae obtained from this crossing were selected for green fluorescence, indicating the presence of the reporter gene CMV:GFP(loxP)LacZ. A–C: A Cre-positive tadpole showing LacZ staining in muscle cells. A: Before LacZ staining, green filter set. B: After LacZ staining, blue myotomes are detected. C: Diffuse staining was observed in the heart. D–F: A Cre-negative tadpole before LacZ staining (D), green filter set. E: After LacZ staining, lateral view. F: After LacZ staining, ventral view. G–I: Close-up of a Cre-positive larvae in the tail showing incomplete staining of the myotomes (G), in the head showing incomplete LacZ staining of the myofibrils of the jaw (H), and in the head region near the eye (I). Examples for unspecific LacZ staining are indicated by arrows. K: Polymerase chain reaction analysis for the presence of the Cre transgene in tadpoles with or without activation of the lacZ reporter gene in muscle cells. wt, wild-type tadpole as negative control. Scale bars = 1 mm in A–H, 100 μm in I.Download figure to PowerPoint Image published in: Waldner C et al. (2006) Copyright © 2006. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View

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