XB-IMG-75398
Xenbase Image ID: 75398
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Fig. 2. Expression levels of XFGFR1 and XFGFR4 are important for normal development of the anterior neural region. (A) The site of microinjection at the 8-cell stage (animal view with dorsal side up) is indicated by the red arrow. (B-I) The embryos were examined for Xpax2 expression (blue) and β-galactosidase activity (red on the injected side). Green arrows indicate Xpax2 expression on the injected side. Xpax2 expression is shifted anteriorly by XFGFR1 mRNA (B left) and XFGFR4-MO (E), and posteriorly by XFGFR4 mRNA (C) and XFGFR1-MO (D). Some of the XFGFR1 mRNA- injected embryos exhibited a miling phenotype(B right). The phenotypes induced by MOs were reversed by the rescue constructs, resXFGFR1 mRNA (F) and resXFGFR4 mRNA (G). The directions of the shifts are determined by the ICDs, as shown by injecting XFGFR1/4 (H) and XFGFR4/1 (I) mRNAs. (J) MO-mediated translational inhibition. UTRXFGFR1-GFP and UTRXFGFR4-GFP are the 5untranslated regions of XFGFR1 (plus the first 4 codons) and XFGFR4 (plus the first 8 codons) fused to GFP, respectively. Whole lysates from stage-11 embryos injected with mRNA (500 pg) and MO (35 ng) were analyzed by western blotting for GFP. myc-GFP mRNA was injected as an injection control. (K) Structures of the wild-type and chimeric XFGFRs. The regions of XFGFR1 and XFGFR4 are shown in red and blue, respectively. (L) Summary of injections. Preprolactin (pplactin) mRNA was injected as a control RNA. Image published in: Yamagishi M and Okamaoto H (2010) Copyright © 2010. Reproduced with permission of the Publisher, University of the Basque Country Press.
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