XB-IMG-75399
Xenbase Image ID: 75399
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Fig. 4. Competition for ligands between XFGFR1 and XFGFR4 regulates the development of the anterior neural region. (A,B) caXFGFR1 mRNA and XFGFR4 mRNA shifted Xpax2 expression anteriorly. (C-F) iXFGFR mRNA shifted Xpax2 expression anteriorly when AP20187 was added at stage 10 to dimerize the iXFGFRs. (G-J) When XFGFR4 mRNA was coinjected, the effect of FGF overexpression (reduced size of the anterior neural structure in a cell-autonomous manner; G,H) disappeared, and Xpax2 expression was shifted posteriorly (I,J). (K,L) ERK assay in animal caps injected with mRNAs encoding caXFGFRs and iXFGFRs. caXFGFR mRNA- and GFP mRNA-injected caps excised from blastulae were incubated at 23oC for 110 min (K). AP20187 was added to iXFGFR mRNA- and GFP mRNA-injected caps at stage 10, and the caps were incubated at 23oC for an additional 110 min (L). Cell lysates were analyzed by western blotting for phosphorylated ERK (pERK), pan-ERK, and GFP. (M) Induction of Xbra expression in animal caps injected with mRNAs encoding caXFGFRs and iXFGFRs. AP20187 was added at stage 9 for dimerization of iXFGFRs. RNA for RT-PCR analysis was extracted at stage 11. (N) Structures of caXFGFRs, iXFGFRs and their derivatives. (O) Summary of injections. Image published in: Yamagishi M and Okamaoto H (2010) Copyright © 2010. Reproduced with permission of the Publisher, University of the Basque Country Press.
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