Xenbase Image ID: 75415

Fig. 4. Effect of overexpression of milk on Xenopus development. (A) milk RNA injection in the ventral marginal zone results in no marked phenotypic effect. (a) Neurula-stage embryos injected with 100 pg of milk RNA in the ventral marginal zone. Note that ventral cells have failed to involute. (b) The same embryos as in a at the tailbud stage. The two embryos have developed apparently normally. (B) milk overexpression in the dorsal marginal zone causes abnormal migration of cells. (a,c,e) Control embryos injected with 500 pg of lacZ RNA. (b,d,f) Embryos injected with 100 pg of milk RNA. (a,b) Late stage gastrula, vegetal view, dorsal is up. (c,d) Neurula stage, dorsal view. (e,f) Late stage neurula, frontal view, dorsal is up. Note that the dorsal cells have failed to involute resulting in an absence of gastrulation and neurulation. (C) Effect of the injection of different doses of milk RNA in the dorsal marginal zone. (a) The top embryo was injected with 500 pg of lacZ RNA. Below, two embryos injected with 100 pg of milk RNA at the tailbud stage show microcephaly. (b) The same embryos as in a at a later stage. (c) Different phenotypes observed in embryos injected with 1 ng of milk mRNA. (d) Dorsal view of an embryo in c; no axial structures have developed. Image published in: Ecochard V et al. (1998) Copyright © 1998. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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