XB-IMG-75416
Xenbase Image ID: 75416
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Fig. 5. Effect of milk injection on Xbra, goosecoid, Xvent-1 and endodermin expression. Overexpression of milk in the dorsal marginal zone results in down-regulation of the early mesodermal markers Xbra and gsc and in the stimulation of edd expression revealed by in situ hybridisation. (A) Xbra expression in an uninjected embryo at stage 11.5. (B) Xbra expression in an embryo injected dorsally with 100 pg of milk plus lacζ mRNA. The cells stained with X-gal do not express Xbra. (C) gsc expression in an uninjected embryo at stage 10.25. (D) gsc expression in an embryo injected dorsally with 100 pg of milk plus lacζ mRNA. The cells stained with X-gal do not express gsc similar to that seen with Xbra. (E) Xvent-1 expression in an uninjected embryo at stage 11.5. (F) Xvent-1 expression in an embryo injected ventrally with 100 pg of milk RNA. Note a repression of Xvent-1 hybridisation signal on the site of milk injection. (G) Embryo coinjected with 100 pg of milk plus lacζ mRNA then stained with X- gal at stage 11.5. (H) The same embryos as in G labelled by an edd- digoxigenin riboprobe. The X-gal-positive cells express edd. edd expression expands outside its normal expression domain (see control J). (I) Embryo injected with 500 pg of milk plus lacζ into one micromere at the 8-cell stage, then stained with X-gal prior to in situ hybridisation for edd at stage 12. Note a patch of edd-expressing cells within the domain of X-gal-positive cells. (J) edd expression in an uninjected embryo at stage 11.5. Image published in: Ecochard V et al. (1998) Copyright © 1998. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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