Xenbase Image ID: 75468

Fig. 1. The role of radial intercalation in ciliated cell differentiation. (A) The development of the two-layered ectoderm into a ciliated epithelium. The outer layer (red) gives rise to epidermal outer cells (OC). Inner layer cells give rise to ciliated cell precursors (CCPs, green) that become ciliated cells (CC) as well as non-ciliated cells (INCs) (Drysdale and Elinson, 1992). (B) The outer layer labeled with mRFP RNA was transplanted at stage 10 onto inner layer labeled with mGFP RNA. At stage 28, embryos were fixed and imaged by confocal microscopy. Cells from the inner layer (green) are either ciliated cells (CC) or non-ciliated (INC). (C) Time-lapse imaging of transplant made as in B. At stage 12, the ventral side of the embryo containing the graft was excised and placed against a coverslip for imaging under low power with a fluorescent microscope. Shown are images taken at the indicated times, from around stage 14 to stage 22. (D-F) Outer layer ectoderm was transplanted onto control host embryos (mGFP) or onto host embryos expressing an inhibitor of Notch signaling (dnHMM). Transplants were fixed at stage 28 and stained with an antibody to acetylated α-tubulin (blue) to identify ciliated cells, and imaged by confocal microscopy. (D,E) Images through the apical surface, identifying outer cells (red, OC), ciliated cells (green/blue, CC) and intercalating non-ciliated cells (green, not blue, INC). (F) The quantification of the three different cell types present in ten fields from two transplants for each condition. Image published in: Stubbs JL et al. (2006) Copyright © 2006. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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