XB-IMG-75471
Xenbase Image ID: 75471
|
|
Fig. 4. Intercalation of excess CCPs. RNA encoding dnHMM was injected intoα -tubulin-mGFP transgenics at the two-cell stage along with mRFP RNA to trace the injected side. At early (stage 18) and late (stage 26) neurulae stages, embryos were fixed and imaged in the confocal microscope. (A-D) Embryos at the designated stages were imaged from the external surface to detect α-tubulin-mGFP-expressing cells that are embedded in the outer layer. (E-H) When the skin is removed and imaged from the internal surface, the number of mGFP-expressing cells located in the inner layer increases significantly in regions expressing dnHMM. At stage 18, the mGFP-expressing cells located internally spread out and extend protrusions (F), but at stage 24 have rounded up and appear poorly attached to surrounding cells (H). (I) Number of GFP-expressing cells that could be imaged apically (outer layer) or basally (inner layer) in α-tubulin-mGFP transgenic embryos at the indicated stage, either in control regions (cont) or regions expressing dnHMM. Image published in: Stubbs JL et al. (2006) Copyright © 2006. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |