XB-IMG-75472
Xenbase Image ID: 75472
Fig. 5. Morphology of INCs, CCPs and outer cells during radial intercalation. Albino embryos transgenic for α-tubulin-mGFP were injected at the two-cell stage with mRFP RNA alone (A,B) or a mixture of mRFP and dnHMM RNA (C-F). (A-D) Transgenic embryos at an intermediate stage in intercalation (stage ∼18) were imaged live in the confocal microscope to score the morphology and number of ciliated cells (mGFP+), outer cells or INCs (asterisks). (A,C) A confocal slice viewed apically; (B,D) a slice 6 μm below the apical surface. broken lines indicate cell-cell contacts between outer cells; white lines indicate outer cell area. (E,F) Confocal images of transgenic embryos at stage 22, showing trapped CCPs (arrowheads) located below the apical surface. Images are a composite of apical mRFP expression and a 6 μm stack of mGFP expression. Scale bars: 10 μm. Image published in: Stubbs JL et al. (2006) Copyright © 2006. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |