XB-IMG-75519
Xenbase Image ID: 75519
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Fig. 2. FoxB1 cooperates with inhibition of BMP signaling to promote commitment to neural fate at the expense of epidermal fate. (A–F) FoxB1 cooperates with the inhibition of BMP signaling (dominant negative BMP receptor, delta-BMPR) to expand the expression of the neural plate marker Sox2 (arrowheads, C) at the expense of epidermal keratin expression (arrowheads, D). Together with beta-galactosidase (beta-gal) mRNA, GR-FoxB1 (250 pg) and delta-BMPR mRNA (125 pg) were injected unilaterally into both dorsal and ventral animal blastomeres of 8-cell stage embryos as indicated. The expression of Sox2 (A, C, and E) and epidermal keratin (B, D, and F) analyzed by in situ hybridization is shown in purple and beta-gal is stained in red. The injected side of the embryo is indicated by brackets. All panels show dorsal views with posterior to the top. (G) FoxB1 and delta-BMPR cooperatively expand the expression domain of Sox2. Summary of phenotypes shown in (A), (C) and (E). Each experiment was repeated more than twice and gave similar results. (H) FoxB1 and delta-BMPR do not cooperatively expand the expression domain of En2. Photographs of stained embryos are not presented. Image published in: Takebayashi-Suzuki K et al. (2011) Copyright © 2011. Image reproduced with permission of the Publisher, Elsevier B. V.
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